oligo deprotection
Martin Leach
leach at mbcrr.harvard.edu
Sun Jun 5 10:03:15 EST 1994
In article <2so70d$6ad at lyra.csx.cam.ac.uk>, arosenth at crc.ac.uk (Dr. A.
Rosenthal) wrote:
> Hi everybody,
> somebody just told me that he had heard, one could deprotect oligos by boiling
them
> for a short while!? rather than the 5hour or o/n 55'C waterbath.
> Any comments greately appreciated.
> Thanks.
Alternatively, u could buy the FOD type phosphoramidites...these deprotect
in an hour...(ABI and Cruachem sell them...not sure what other companies
do..). Have used these...they work fine.
Martin
--
.... Martin Leach Email:leach at mbcrr.harvard.edu
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