RNase inhibitor

Damak, Sami damak at kahu.lincoln.ac.nz
Wed Jun 8 01:11:20 EST 1994


In article <2sifmn$e39 at gazette.bcm.tmc.edu> wzhao at phy.bcm.tmc.edu writes:
>From: wzhao at phy.bcm.tmc.edu
>Subject: RNase inhibitor
>Date: 1 Jun 1994 17:15:03 GMT

>Hi, netters:

>I have been doing in vitro transcription by T7 polymerase recently.
>I am wondering if the RNase inhibitor is critical or essential 
>for the transcription. Any advice is greatly appreciated.

>Wei

Every now and then, I run out of RNAse Inh and do the transcription without 
it.  It has never failed.  I guess if standard RNA precautions are taken, 
there is no need for it.

Sami



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