Pfu-spiked cycle sequencing

Robert Preston rapr at MED.PITT.EDU
Wed Jun 8 08:48:57 EST 1994

Forwarded message:
> From: jspaffor at (J. David Spafford)
> [edit] 
> I use an ABI sequencer with a 9600 Perkin Elmers PCR machine.  Usually I can
> get up to 400 bp of good sequence and no more.  ABI provides AmpliTaq with
> their sequencing kit.  Would I get longer sequence if I spike it with Pfu
> DNA polymerase?
> J. David Spafford

You mean, do Wayne Barnesian Long-PCR factors come into play in the 400-700
bp range?  Interesting possibility.  Well worth an experiment.  Be sure to
post the results!

Rob Preston
rapr at

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