Pfu-spiked cycle sequencing
rapr at MED.PITT.EDU
Wed Jun 8 08:48:57 EST 1994
> From: jspaffor at gpu.srv.ualberta.ca (J. David Spafford)
> I use an ABI sequencer with a 9600 Perkin Elmers PCR machine. Usually I can
> get up to 400 bp of good sequence and no more. ABI provides AmpliTaq with
> their sequencing kit. Would I get longer sequence if I spike it with Pfu
> DNA polymerase?
> J. David Spafford
You mean, do Wayne Barnesian Long-PCR factors come into play in the 400-700
bp range? Interesting possibility. Well worth an experiment. Be sure to
post the results!
rapr at med.pitt.edu
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