Surf Blot Experience
ed_beaty at qms1.life.uiuc.edu
Thu Jun 9 11:37:22 EST 1994
In article <22 at abr.win.net>, jstiehr at abr.win.net (James Stiehr) wrote:
> Dear Netters:
> I would appreciate hearing from anyone who has had experience using
> devices that can be clamped over a Western blot to create lanes
> which allows one to use different primary antibodies on the same
> blot. Idea Scientific Co. has their "Surf Blot" which does this.
> Do these devices work; are there problems with leaks between
> channels; are they difficult to work with? I would appreciate any
> comments or anectdotes from experienced users of these things.
> Also, does anyone know of a good source for inexpensive pre-poured
> gels for a Bio-Rad mini-gel system.
> James Stiehr
> jstiehr at abr.win.net
This sounds neat, but is it really necessary? It sound like it would be a
lot cheaper to cut up your blotted membrane and perform the blots in 4 or 5
different trays, each with a different antibody. (Being sure to label each
Just a thought.
Ed_Beaty at qms1.life.uiuc.edu
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