Pfu-spiked cycle sequencing

Duncan Clark Duncan at genesys.demon.co.uk
Thu Jun 9 10:02:24 EST 1994


Surely spiking a sequencing reaction with a proof-reading polymerase like
Pfu will give poorer results 'cos the Pfu will remove the dideoxy terminator?
Obviously it will depend on how much Pfu is there but even at the Barnes
ratio I would still think the Pfu would remove the terminator. I hope I'm
wrong so please try it.

Duncan

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Duncan Clark                        | Internet:    duncan at genesys.demon.co.uk
GeneSys Ltd.                        | Compuserve:  100015.1406 at compuserve.com
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