Expression from Bluescript

BSS032 at BANGOR.AC.UK BSS032 at BANGOR.AC.UK
Fri Jun 10 01:53:01 EST 1994


Regarding expression from the T7 promoter in Bluescript.

We have had good levels of expression from Gibco's pT7T3 which is (as far as
I can work out) more or less the same as Bluescript. We used JM109(DE3)
which carries the T7 pol gene on the DE3 prophage (available from Promega).

Hope this helps.

Jon R. Sayers

Univ. of Wales, Biochemistry, Bangor, Gwynedd, Wales, UK.

+(44) 248 38 23 54

Email bss032 at bangor.ac.uk



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