in vivo protein expression from pBluescript

Suzanne Patterson spatters at ccs.carleton.ca
Thu Jun 9 20:59:57 EST 1994


I would like to know if it's possible to get transcription of
foreign genes, in vivo, from the T7 promoter in the pBluescript phagmid
using an E coli strain containing the T7 RNA polymerase gene like the
strains used with the novogen pet vectors?  When I asked stratagene, they
said they had not tried it but that other people had asked about it, so
someone has to have tried it. Did it work?  Any problems or suggestions?
Thanks, Suzanne Paterson.



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