non-radioactive translation kits

Frances Hannan Zoology flh at
Thu Jun 9 07:23:35 EST 1994

Has anyone tried the new non-radioactive in vitro translation kits 
from Promega or Boehringer? Briefly these use a biotinylated amino 
acid instead of radioactively labelled. Which reticulocyte lysates
have been tried (since some apparently contain biotinylatable proteins
that can contribute to background)? I will summarize any replies.

*   Frances Hannan                                       *
*   BILMS, Zoology, Downing St, Cambridge, CB2 3EJ, UK   *
*   Phone (0223)336663, FAX (0223)461954                 *
*   flh at                               *

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