PCR for Random Mutagenesis - Help!

[Steve Minchin]

In article <0097FB3C.3979C440.440 at umbsky.cc.umb.edu> 5978jsnyd at UMBSKY.CC.UMB.EDU writes:
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>978jsnyd
>From: 5978jsnyd at UMBSKY.CC.UMB.EDU
>Newsgroups: bionet.molbio.methds-reagnts
>Subject: PCR for Random Mutagenesis - Help!
>Date: 9 Jun 1994 13:21:28 -0700
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>Hello to all -

>I'm trying to find a procedure that uses PCR to cause random mutations 
>in DNA as it is amplified.  Has anyone done this, or does anyone know 
>where I can find a protocol for this procedure?  Any help would be 
>appreciated.


>John Snyder
>UMass/Boston Biology Department


Hi John,

We have used PCR mutagenesis quite successfully, but we do have a good 
selection/screening protocol to determine the mutants we want.
There is no single protocol, since the protocol will depend on the size of 
your PCR product and the number of mutants per product required.

There is a good review of PCR conditions and fidelity by Eckert and Kunkel (pg 
225-244)  in the book:

PCR - A Practical Approach

Edited by McPherson, Quirke and Taylor

IRL Press at Oxford University Press.



All the Best

Steve Minchin



---------------------------------------------------------
Dr. Steve Minchin,

School of Biochemistry, The University of Birmingham,
Edgbaston, Birmingham, B15 2TT, U.K.

E-Mail
InterNet:       S.D.Minchin at bham.ac.uk
Janet:          S.D.Minchin at uk.ac.bham

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