Buffering Phenol: The Light Dawns
Shiao Y. Wang
sywang at whale.st.usm.edu
Sat Jun 11 13:46:30 EST 1994
: OK, phenol phans, here is the definitive answer:
: I was taught by a former PhD student (Leona Fitzmaurice) from Alan
: Williamson's lab in Scotland. [deleted]
: Best results for DNA came from phenol prepared the way she taught me.
: That is: equilibrate (shake) the phenol against 0.1M TrisHCl, pH8, and
: let the phases separate.
What about the 3 drops of 5 M NaOH per 10 ml phenol + Tris that you
suggested in an earlier posting? Please clarify and be consistent.
University of Southern Mississippi
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