Buffering Phenol: The Light Dawns

Shiao Y. Wang sywang at whale.st.usm.edu
Sat Jun 11 13:46:30 EST 1994


: OK, phenol phans, here is the definitive answer:

: I was taught by a former PhD student (Leona Fitzmaurice) from Alan
: Williamson's lab in Scotland. [deleted]
:  Best results for DNA came from phenol prepared the way she taught me. 
: That is: equilibrate (shake) the phenol against 0.1M TrisHCl, pH8, and
: let the phases separate. 

What about the 3 drops of 5 M NaOH per 10 ml phenol + Tris that you
suggested in an earlier posting? Please clarify and be consistent.

Shiao Wang
University of Southern Mississippi



More information about the Methods mailing list