in vitro transcription: low incorporation of label
bawagan at umdnj.edu
Sat Jun 11 08:53:24 EST 1994
I am posting this for an acquaintance who mentioned the problem to me.
He needs to make an RNA probe from a cDNA template by run-off transcription
in vitro. The RNA made incorporates UTP (then he tried CTP) at a very low
efficiency of 1% while the DNA template of the Maxi Script kit produces RNA
with a 50% incorporation. He suspects "rearrangement of some sequences in
the cDNA template during the subcloning which causes early termination of
What do you think? Thank you.
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