To Pichia or not to Pincha??
damak at kahu.lincoln.ac.nz
Sun Jun 12 07:47:34 EST 1994
In article <33191.M.Holland at dwe.csiro.au> M.Holland at dwe.csiro.au ("Michael Holland") writes:
>From: M.Holland at dwe.csiro.au ("Michael Holland")
>Subject: To Pichia or not to Pincha??
>Date: 9 Jun 1994 17:14:20 -0700
>Have I missed a decent discussion of the merits/demerits of this system? I
>have several VERY heavily glycosylated glycoproteins that I wish to express
>with a near "natural" glycosylation as I can get. I won't get enough protein
>from a vaccinia system so it seems baculovirus or picha are my choices. Any
>comments on the relative glycosylation abilities of the two systems, ease of
>use, yiels etc would be appreciated and summarised for the record.
>Michael K Holland PhD
>CSIRO, Wildlife & Ecology
>PO Box 84, Lyneham ACT 2602, Australia
>Fax: 61-6-242-9242 Phone:61-6-242-1793
>mholland at dwe.csiro.au
Have you considered expressing your proteins in the mammary gland of
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