To Pichia or not to Pincha??

Damak, Sami damak at kahu.lincoln.ac.nz
Sun Jun 12 07:47:34 EST 1994


In article <33191.M.Holland at dwe.csiro.au> M.Holland at dwe.csiro.au ("Michael Holland") writes:
>From: M.Holland at dwe.csiro.au ("Michael Holland")
>Subject: To Pichia or not to Pincha??
>Date: 9 Jun 1994 17:14:20 -0700

>Netpeople,

>Have I missed a decent discussion of the merits/demerits of this system? I
>have several VERY heavily glycosylated glycoproteins that I wish to express
>with a near "natural" glycosylation as I can get. I won't get enough protein
>from a vaccinia system so it seems baculovirus or picha are my choices. Any
>comments on the relative glycosylation abilities of the two systems, ease of
>use, yiels etc would be appreciated and summarised for the record.

>Thanks

>Michael
>Michael K Holland PhD
>CSIRO, Wildlife & Ecology
>PO Box 84, Lyneham ACT 2602, Australia
>Fax: 61-6-242-9242  Phone:61-6-242-1793
>mholland at dwe.csiro.au

Have you considered expressing your proteins in the mammary gland of 
transgenic animals?

Sami



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