Doubletake mutagenesis kit (Stratagene)
mblse at alf.uib.no
Mon Jun 13 10:02:42 EST 1994
Sorry if this is a faq, but Im new here.
Ive been trying to use Stratagene´s Doubletake mutagenesis kit ,
immobilizing a 4,6 kbp 3´-end biotinylated template on the avidine-coated
This far I have not succeeded getting any colonies at all,not even from
the positive control supplied in the kit after transformation of E.coli XL1
Im hoping to hear frome someone who has used the kit.
Thanks in advance !
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