Doubletake mutagenesis kit (Stratagene)

Sigrid Erdal mblse at
Mon Jun 13 10:02:42 EST 1994

Sorry if this is a faq, but Im new here.

Ive been trying to use Stratagene´s Doubletake mutagenesis kit , 
immobilizing a 4,6 kbp 3´-end biotinylated template on the avidine-coated 
latex particles.
This far I have not succeeded getting any colonies at all,not even from 
the positive control supplied in the kit after transformation of E.coli XL1 
Im hoping to hear frome someone who has used the kit.

Thanks in advance !

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