Equilibrium dialysis ? Apparatus/method

U196CFA at CSI.IT U196CFA at CSI.IT
Mon Jun 13 05:28:43 EST 1994



Hi, Darren

Some bibliographic references:
Methods in Enzym. Womack and Colowick (year approx. 1973, p.464) Capter 17 
                  "Rapid measurement of binding of ligands by rate of dialysis"
Examples: Biochim.Biophys. Acta 55 (1962) 361-373
          Biochim. Biophys. Acta 536 (1978) 18-26
          Agric. Biol.Chem. 49 (1985) 2125-2130 or
          J. Biochem. 105 (1989) 444-448 (comparison among equil.dialysis, 
          fluorescence and UV-difference spectroscopy) 
          Proc. Natl.Acad.Sci. USA 73 (1976) 361-365


Equipment:
dialysis cells :
Six different types from Scienceware (Bel-Art Prod. USA)
A equilibrium microvolume dialyzer from Hoefer Sci.Instr. 654 Minnesota Str.
box 77387, San Francisco, CA 94107-0387. fax 415-821-1081

Our results:
good and reproducible results using both radioactive and dye binding agents. 

Bye

Franco Dosio
Ist.Chim.Farm.Applicata
University of Turin, Italy
dosio at silver.ch.unito.it
u196cfa at csivms.csi.it



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