PCR and BrdU

K.M.Toellner at bham.ac.uk K.M.Toellner at bham.ac.uk
Mon Jun 13 09:12:38 EST 1994


We are planning, to perform PCR from cells, which are treated for 3h with 
BrdU.
Does anybody know, whether Bromodeoxyuridine in the DNA-template 
inhibits the PCR? And if so, whether the PCR-protocol has to be altered?

Kai-M. Toellner   
Dept. of Immunology     
The Medical School                                                 
University of Birmingham                             
Birmingham B15 2TT, UK                      
E-mail: K.M.Toellner at bham.ac.uk



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