Buffering Phenol: pH directly
jgraham at bronze.ucs.indiana.edu
Mon Jun 13 13:37:15 EST 1994
Jim Owens writes:
> I have heard that it is possible to measure the pH of an organic solution,
> but a pH meter or the pH papers will not give an accurate measure.
It is indeed.
Mix 2 ml of organic phase with 8 ml of methanol and 10 ml of water. Use
a pH meter.
Mix 2 ml of organic pahse with 5 ml methanol and 13 ml of water.
I think the problems mentioned contribute greatly to the variability of
organic extractions. I prefer to substitute a Proteinase K digest for
phenol extraction whenever feasible.
J. E. Graham
Biology and Chemistry Departments
Indiana University Bloomington
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