Anchor PCR question
corell at u.washington.edu
Mon Jun 13 13:34:45 EST 1994
I am about to do some anchor PCR. The target RNA may be relatively
short compared to the first strand oligo primer (not oligo-dT). I need
to get rid of the first strand primer before I can efficiently tail
my cDNA for the anchor PCR. Since I can't rely on differential
precipitation based on size, I need a way to get rid of the oligo
without losing my cDNA. Any ideas? References please...
I've heard there is a way to precipitate double-stranded species (i.e.
the cDNA/RNA duplex) without the single stranded oligonucleotide
primers? Is that true?
Thanks for your help.
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