Overcoming Sequencing Secondary Structure

Michael van Waes bi__mvw at SELWAY.UMT.EDU
Mon Jun 13 11:53:29 EST 1994


>
>USB sells c7dGTP mixes in the kit, but a much more effective mix replaces 
>*both* dGTP *and* dATP with their c7 (7-deaza) analogs. You will need to 
>replace the dGTP on a per-mole basis with c7dGTP; and also replace 
>the dATP with c7dATP on a per-mole basis. You will need to do this in both 
>the Labelling and Termination mixes. You will also only be able to use a 
>"c-minus" labelling mix (requiring hot dCTP).
>
<stuff deleted>
>
>Really, this stuff works great!
>
>
>
Do you know if RT takes up the deaza-NTPs?



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