reusing agarose gels
dsammata at magnus.acs.ohio-state.edu
Tue Jun 14 07:47:21 EST 1994
Hello fellow RAPDs people.
I am working with honey bees, spiroplasma and mites and have found
a good protocol (when the RAPDs god/goddess are cooperating) to
extract the DNA using Longmire's bird blood protocol and dialysis
plus salt precipt.
Recently, some of my primers seem to be failing since I have just
purchases new reaction mixes, made my own MgCl and use tissue cultured
water. I also UV pipettors, tips, and tubes as well as the water
to get rid of negative control bands.
A few questions:
1.Can I reuse agarose gels, and what happens to DNA and EB in the
2. What is a positive control?
As my system is shutting down in a few minutes I will sign off
Diana Sammataro, Dept Entom. dsammata at magnus.acs.ohio-state.edu
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