dissection and RNA extraction
Damak, Sami
damak at kahu.lincoln.ac.nz
Tue Jun 14 06:41:05 EST 1994
We need to extract RNA from sheep sweat glands. The problem is that it
takes two hours to dissect enough glands to work with, by which time the RNA
is completely degraded. We tried fixing the tisssue or dissecting in tissue
culture medium but still didn't get any success. We use the Trizol method
to extract RNA.
Does anyone have any experience with extracting RNA from tissues that are
difficult to dissect? Any suggestion will be appreciated.
Sami
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