dissection and RNA extraction

Damak, Sami damak at kahu.lincoln.ac.nz
Tue Jun 14 06:41:05 EST 1994


We need to extract RNA from sheep sweat glands.  The problem is that it 
takes two hours to dissect enough glands to work with, by which time the RNA 
is completely degraded.  We tried fixing the tisssue or dissecting in tissue 
culture medium but still didn't get any success.  We use the Trizol method 
to extract RNA.
Does anyone have any experience with extracting RNA from tissues that are 
difficult to dissect?  Any suggestion will be appreciated.

Sami



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