Tth 111 II

[Duncan Clark]

In article: <2tir0u$6dd at gazette.bcm.tmc.edu>  lr035778 at mbcr.bcm.tmc.edu (Laurence T. Reiter) writes:
> 
> So I was doing this restriction mapping on some sequence which I just got and
> I found the perfect enzyme which would be most informative on a Southern of 
> genomic DNA.  The computer cheerfully belched out: Tth 111 II!  What?  Yup.
> Tth 111 II is so special a restriction enzyme, in fact, that it is not 
> commercially available.  THUS:
> 
> HELP!  Does anyone out there know where I can get Tth 111 II?
> 
> Thanks,
> LTR
> Baylor Coll. of Med.
>  

It is so special 'cos the yield is lousy. I can't lay my hands on the 
published paper (misfiled as normal) but compared to Tth111 I it is 
,from memory, down by a factor of at least 250. You will find the 
source of the strain listed in REBASE if you want to try it yourself.

Duncan
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Duncan Clark                        | Internet:    duncan at genesys.demon.co.uk
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