PCR on whole yeast cells

MHAMILTO at vm.ucs.UAlberta.CA MHAMILTO at vm.ucs.UAlberta.CA
Wed Jun 15 00:03:33 EST 1994


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From: dak at apldbio.com (Dave Knorr)
Newsgroups: bionet.molbio.methds-reagnts
Subject: Re: PCR on whole yeast cells
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Date: 13 Jun 94 19:56:50 GMT
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In article <2t7ks6$a9a at mserv1.dl.ac.uk> Dr. I. Dunham, idunham at crc.ac.uk
writes:
>
>>Incidently Eric Green has recently found that this colony PCR does not
>>work
>>reproducibly in thermocylcers with a heated top plate if you omit the
>>mineral oil.

>Does anyone have a theory as to why that would be?

>Dave Knorr


Just a guess, but possibly the mineral oil is sequestering some hydrophobic
compound which bothers the PCR reaction.  My guess would be cell membrane.

Mikey





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