DNA extraction from P. fluorescens

[Ron Caspi]

Hello Netters

I am working with a strain of Pseudomonas fluorescens, and when I try to 
extract chromosomal DNA, I am faced with large amounts of polysaccharides, 
that make life much more complicated. Does anybody have suggestions, either a 
different growth medium or a protocol that will solve the problem?

Ron

Ron Caspi  
Scripps Institution of Oceanography      Phone (619)534-0638
La Jolla  CA  92093-0202                 Fax   (619)534-7313