PKA phosphorylation in vitro

Peter Uetz uetz at embl-heidelberg.de
Tue Jun 14 14:17:33 EST 1994


Has anybody tried to in vitro phosphorylate PKA-tagged proteins ?

I was told that PKA from Sigma works only in 3 out of 10 cases.
(My batch did'nt).

Who made the same experience with Sigma PKA ?

Which PKA (company) is recommended ?



Thank you for any advice,


peter uetz  (UETZ at EMBL-Heidelberg.de)




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