Long PCR smear

mhollowa at epo.som.sunysb.edu mhollowa at epo.som.sunysb.edu
Tue Jun 14 12:46:17 EST 1994


In article <94163.093714ABW1 at psuvm.psu.edu> Anne Buchanan <ABW1 at psuvm.psu.edu> writes:
> we removed a tube
>from the thermal cycler every 5 cycles to check the effects of number of
>cycles, and the only difference more cycles made was longer and longer smear.

I can't claim much experience with PCR, but I'll put in my two 
cents anyway.  With such a large number of cycles, isn't it 
possible that you're seeing an increase in incomplete strands?  
Perhaps a survey of clone lengths would be a better indicator 
of success. (?)

Mike Holloway
mhollowa at epo.som.sunysb.edu




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