hygromycin phosphotransferase
Glenn Cantor
GCANTOR at VETMED.WSU.EDU
Wed Jun 15 18:51:37 EST 1994
I have transfected cells with a plasmid containing a hygromycin
selectable marker. To compare transfection efficiency in various
cell lines, I would like to compare the amounts of hygromycin
phosphotransferase which the lines express. Does anyone know if
antibodies or an antibody-based kit are available for detection of
hygromycin phosphotransferase? (5'-3' has an ELISA for detection of
neomycin phosphotransferase II, but doesn't have one for hygromycin.)
Thanks!
Debra Hoover
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