Quenching FITC

[Michael J. Tino]

[ Article crossposted from bionet.immunology ]
[ Author was Michael J. Tino ]
[ Posted on 15 Jun 1994 21:52:34 GMT ]

I am having trouble with an assay for phagocytosis in which I would like to 
examine fluorescence of ingested FITC-labelled bacteria by macrophages.
In order to do this, I must quench the FITC outside of the macrophages to
distinguish between extra- and intracellular fluorecence.  I've
tried crystal violet and trypan blue, but with no success.  Any suggestions?

Michael Tino
michael_tino at cellbio.duke.edu

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                              ______
Michael James Tino            \    /   "the only measure of your words
Duke University                \  /    and your deeds  will be the love
Department of Cell Biology      \/     you leave behind when you're done."
                                         -Fred Small, "Everything Possible"
mjtino at acpub.duke.edu
michael_tino at cellbio.duke.edu

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--


**************************************************************************
                              ______
Michael James Tino            \    /   "the only measure of your words
Duke University                \  /    and your deeds  will be the love
Department of Cell Biology      \/     you leave behind when you're done."
                                         -Fred Small, "Everything Possible"
mjtino at acpub.duke.edu
michael_tino at cellbio.duke.edu

**************************************************************************