Vent PCR Woes

the End jgraham at bronze.ucs.indiana.edu
Wed Jun 15 13:22:40 EST 1994


I agree with Tony, Vent does outperform Taq, however try to keep the 
enzyme away from your primers and template except for the actual 
"reaction". Also keep everything on ice prior to starting to prevent 
Vent exonuclease activity.


Jim
J. Graham
..............."Licence ? It's back there"........... Cheech and Chong



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