quantifying of nucl. acids

at umich.edu
Wed Jun 15 13:04:20 EST 1994

In article <12JUN199416010095 at opal.tufts.edu>, jpalek at opal.tufts.edu (HYNEK

> Hi everybody,
> I have to quatify in vitro synthesized RNA for oocyte injection.  
> I have tried to take OD, but numbers, I am getting look very inaccurate.
> Do you have any suggestions (besides comparing intensity of bands in agarose
> gel) for very fine measurement of amount of nucleic acides?

RNA forms crystals when precipitated and is often difficult to dissolve.
Use a pipet tip or heat to 65 degrees to breakup the pellets.

Steve Coon
sdcoon at umich.edu
University of Michigan School of Dentistry

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