hygromycin phosphotransferase

David Huen s.d.huen at bham.ac.uk
Thu Jun 16 04:37:51 EST 1994


In article <1994Jun16.055925.2471 at alw.nih.gov>, bernard at elsie.nci.nih.gov
(Bernard Murray) wrote:

<stuff deleted>

> 	While we are on the topic, has anyone any experience of cells
> becoming hygromycin resistant without transfection with this gene?  I believe
> that G418 is not a substrate for classic multidrug resistance (pgp/P170/mdr)
> but I do not know if hygromycin has been tested.
> 		Bernard

Well, it's not quite the answer you are looking for but there are B-cell
lines in this lab which require 3 mg/ml G418 selection. Not even yeast can
contaminate those blasted cultures. Conversely, we'd go broke if we had to
have them out of nitrogen for long....



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