Ligation of PvuI site

Bulletin board login maryr at
Thu Jun 16 06:32:43 EST 1994

I have been trying to set up a plasmid construct using a Pvu I site.  This 
site does not want to ligate.  I've tried BRL PvuI with no success but, have
had a little bit of success with PVuI from New ENg. Biolabs but, no success 
lately even with NEB enzyme.  Any help or suggestions would be greatly 
appreciated.  Regards, Mary

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