Very large Plasmid Preps

Roger Graham flo at smtp.unixg.ubc.ca
Fri Jun 17 19:43:05 EST 1994


Netters,

What protocol is best for doing very large plasmid preps in E. coli, e.g.
>200 g wet cell weight ? Do people just keep scaling up alkaline lysis ? Is
there a more efficient way ? Thanks for input.

-- 
Roger Graham
flo at unixg.ubc.ca
Dept. of Microbiology
University of British Columbia
Vancouver, B.C., Canada
Yes it is raining here.



More information about the Methods mailing list