Very large Plasmid Preps

Roger Graham flo at
Fri Jun 17 19:43:05 EST 1994


What protocol is best for doing very large plasmid preps in E. coli, e.g.
>200 g wet cell weight ? Do people just keep scaling up alkaline lysis ? Is
there a more efficient way ? Thanks for input.

Roger Graham
flo at
Dept. of Microbiology
University of British Columbia
Vancouver, B.C., Canada
Yes it is raining here.

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