RNAse stability

Shahram Mori smori at nmsu.edu
Tue Jun 14 03:32:13 EST 1994


David Kling (dkling at warren.med.harvard.edu) wrote:
: Hi netters,

: Does anybody know if RNAses are susceptible to freeze thawing 2 or 3 times?

: Thanx,

: David Kling
This really depends on the organism, but generally they are very sturdy. Very
adverse conditions must be done in order to inactivate them. For example
Autoclaving alone does not kill the RNases. I use procaryotic organisms in
which RNases are not inactivated by continuous freeze thawing.
Cheers,
Shahram Mori
Program in Molecular Biology
Dept. of Chemistry and Biochemistry
NMSU, las Cruces NM
88003

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