PCR mutagenesis

[Michael Benedik]

Does someone have the protocol/reference for doing random mutagenesis
by PCR amplification of a target fragment. I think you just change
nucleotide concentrations and/or add Mn++, but I don't have the details.

Thanks.

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 Michael Benedik				INTERNET: Benedik at uh.edu
 Dept. of Biochemical & Biophysical Sciences	
 University of Houston				BITNET: Benedik at uhou
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