Remove CH3CN and other solvent from small RNA (4mer)

dxy at dxy at
Fri Jun 17 22:28:09 EST 1994


It sounds sample but a trouble for me. 

CH3CN (acetonenitrile) should go away easily but after dry the 4mer 2 or 3 times I 
still see strong signal of CH3CN in NMR spectrum. 

I tried ethanol ppt, then dry it, still CH3CN is there.

The 4mer has a MW about 1000, I have no idea  what gel filtration matrix will work.

Some solvents or salt have to be used in geting the 4mer, then comes the problem
how to remove them. 




More information about the Methods mailing list