Optimal E Coli strains for MBP expression

Michael Holland M.Holland at dwe.csiro.au
Sat Jun 18 23:34:30 EST 1994


Nettpeople,

We are currently using XL-1 Blue to express a clones gene from rabbit sperm.
The rate of production of recombinant protein is not great at 1 ug/ml
culture and in addition we see an number of breakdown products which are
being formed in the bacteria not during purification. Other people here who
 have expressed other (unrelated) sperm proteins also see the breakdown
 problem. What other coli strains are appropriate? What ae the advantages
and disadvantages?

Thanks
Michael K Holland PhD
CSIRO, Wildlife & Ecology
PO Box 84, Lyneham ACT 2602, Australia
Fax: 61-6-242-9242  Phone:61-6-242-1793
mholland at dwe.csiro.au



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