Hyg, Neo, and $$

David Huen s.d.huen at bham.ac.uk
Sat Jun 18 03:28:06 EST 1994


In article <s.d.huen-170694084521 at med242.bham.ac.uk>, s.d.huen at bham.ac.uk
(David Huen) wrote:
> 
> In article <1994Jun17.000602.4613 at emba.uvm.edu>, brianf at med.uvm.edu (Brian
> Foley) wrote:

> > 	Yes, the bottle of hygromycin I got from Calbiochem says it is 
> > suspected of being carcinogenic and a teratogen.  This would cause you
> > to beleive that it is also mutagenic.  The only thing I have done 
> > about this is to transfect a control lot of cells with the same plasmid
> > carrying no insert DNA.  These cells are maintained on hygromycin
> > right beside the cells transfected with the plasmid carrying my gene
> > of interest.  I have not yet seen any mutations in the system I screen for.
> 
> Since hyg targets the nucleotide synthesis pathway, it could potentially be
> mutagenic.

Sorry, Dr. Foley is correct. hygromycin is a elongation inhibitor. The one
I am thinking of is mycophenolic acid, which we use here as well.



More information about the Methods mailing list