RNA isolation request

[Joseph C. Bagshaw]

For total RNA, try the guanidinium/acid phenol method of Chomcyynski
and Sacchi, Anal. Biochem. 162, 156-159, 1987.  You can also find this 
in Current Protocols in Mol Biol (a.k.a. the Big Red Book), p. 4.2.4.
You should be able to find someone at NCSU that has it.  If you're
on the Raliegh campus, try Matt Andrews in Genetics.  One thing I
would suggest is to freeze the little buggers in LN2 and grind them
to a powder before you begin the extraction.  Good luck.


In article <2tqe4a$h34 at taco.cc.ncsu.edu> skantar at unity.ncsu.edu (Andrea M Skantar) writes:
>Hi,
>
>I'm posting for a friend who is exploring different protocols for either 
>mRNA isolation or total RNA isolation from nematodes (specifically plant 
>parasitic nematodes).  If anyone can direct us to some good (or bad) 
>protocols (endorsements or flames welcome), please email me and I'll pass 
>them along.  Or if you know of where in IUBIO archive to look.  I 
>browsed, but did not find anything.  
>
>Thanks,
>
>Andrea


-- 
********************  HAVE GENES, WILL TRAVEL  ********************
Joe Bagshaw, Worcester Polytechnic Institute
jbagshaw at wpi.wpi.edu
Roadkill on the information superhighway.