problems with PCR
WMGDDM at CARDIFF.AC.UK
Mon Jun 20 10:59:35 EST 1994
I've got a problem with a PCR that I'm attempting...
I'm trying to use a pair of primers to follow a series
of deletions that I'm making in a plasmid.
Under 'standard' conditions I can amplify the deleted
version of the plasmid but not the original undeleted
Can anyone explain this, I've tried all the usual
variations in the reaction conditions, etc. with no luck!
Dave Morgan Inst. Medical Genetics
University of Wales College of Medicine
Heath Park, Cardiff. CF4 4XN
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