SDS-PAGE-resistant protein complexes
Alexander P Rostovtsev
rosto001 at maroon.tc.umn.edu
Mon Jun 20 14:28:45 EST 1994
In <Mailstrom.1.03.12087.15089.agibson at acs.ucalgary.ca> agibson at ACS.UCALGARY.CA (Allan Gibson) writes:
>Hi! Has anyone on the net ever had experience with protein complexes that are
>not dissociated under normal SDS-PAGE conditions, i.e. brief boiling in SDS
>sample buffer containing 2% SDS and 5% 2-mercaptoethanol. If so, I'd appreciate
>very much hearing from you and finding what conditions, if any, might break such
>a complex into its components. Thanks.
Don't boil samples. Try 30-45 min incubation at 60-70oC or
overnight at RT.
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