Autoclaving DNA and contamination

Scrimshaw, Brian J SCRIMSHAWB%GRIEHFS at LINCOLN.CRI.NZ
Mon Jun 20 23:32:37 EST 1994


Has anyone done any experiments, or know of any publications,
about what autoclaving does to DNA? I remember being told that
autoclaving shears DNA, but not below 150bp, I have never seen a
reference on this though.

Since many PCR products are around this size, this would mean
autoclaving would not eliminate any small contaminating
fragments.

Can someone give me some advice on this?

Brian Scrimshaw
Wellington
New Zealand



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