SDS-PAGE-resistant protein complexes

Allan Gibson agibson at ACS.UCALGARY.CA
Mon Jun 20 13:26:01 EST 1994

Hi!  Has anyone on the net ever had experience with protein complexes that are
not dissociated under normal SDS-PAGE conditions, i.e. brief boiling in SDS
sample buffer containing 2% SDS and 5% 2-mercaptoethanol. If so, I'd appreciate
very much hearing from you and finding what conditions, if any, might break such
a complex into its components.  Thanks.   


More information about the Methods mailing list