DNA extraction from P. fluorescens
Sebastian W. Bunka
seb at i102pc1.vu-wien.ac.at
Tue Jun 21 03:45:52 EST 1994
Ron Caspi (rcaspi at ucsd.edu) wrote:
: Hello Netters
: I am working with a strain of Pseudomonas fluorescens, and when I try to
: extract chromosomal DNA, I am faced with large amounts of polysaccharides,
: that make life much more complicated. Does anybody have suggestions, either a
: different growth medium or a protocol that will solve the problem?
I've never tried it w/ Ps. fluorescens, but several people from
the Univ. Vienna-Biocenter grow capsulated/polysaccharide rich
bacteria with the addition of 100 microMol 2,2' Dipyridil (Bipyridine)
(Sigma D-7505 or other...).
Polysaccharide capsule is reduced, but growth is slower (iron-chelator).
It works with Pasteurella multocida and others.
[ Sebastian.Bunka at vu-wien.ac.at ]
Location: earth, europe, austria, vienna Inst. of Bacteriology Vet.Univ.
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