color selection with omega subunit?

Darrick Carter carterd at news.ohsu.edu
Tue Jun 21 21:23:50 EST 1994


I've got an E. coli strain with a delta lac. i.e. no lacZ
So I can't use blue color selection on complementation with only the
alpha subunit (with eg bluescript) Does anyone know a way to get around
this (like for example a plasmid I could use to transform this strain
before transforming with Bluescript?)
Or, is there a way I can color bacterial colonies on a petri dish, after
they have grown?

Thanks in advance, Darrick Carter (carterd at ohsu.edu)



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