PCR mutagenesis

Elmer M. Price, Ph.D. DCRCEP at mizzou1.missouri.edu
Mon Jun 20 16:23:15 EST 1994


In article <2tsoar$n17 at masala.cc.uh.edu>
bchs1b at Elroy.UH.EDU (Michael Benedik) writes:
 
>
>
>Does someone have the protocol/reference for doing random mutagenesis
>by PCR amplification of a target fragment. I think you just change
>nucleotide concentrations and/or add Mn++, but I don't have the details.
>
>Thanks.
>
>----------------------------------------------------------------------
> Michael Benedik                                INTERNET: Benedik at uh.edu
> Dept. of Biochemical & Biophysical Sciences
> University of Houston                          BITNET: Benedik at uhou
>-----------------------------------------------------------------------
 
 
 
 
 
Michael
     Try "Technique-A Journal of Methods in Cell and Molecular Biology
Vol. 1, No. 1 pp11-15 (1989)", by Leung et al.  We have used the protocols
in this paper with very nice results.
 
Hope this helps
 
Elmer



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