Removing Bind Silane from plates

Cornelius Krasel krasel at alf.biochem.mpg.de
Sat Jun 18 11:47:00 EST 1994


Sean David Moore (smoore at mail.sas.upenn.edu) wrote:

: The bind Silane went through the gel and stuck..in the absence of a
: solvent such as acidic etoh and placed a film on the other plate?

:   Are you sure you didn't place a wet plate on the other before it set?..I
: have never haerd of such a thing...please inform.

It appears that the binding silane does indeed diffuse from the treated
place through the gel and causes binding of the gel to the other plate.
This problem is especially prevalent if you pour the gel the day before
you use it.

Sorry, no physical or chemical explanation.

--Cornelius.

--
/* Cornelius Krasel, Abt. Lohse, Genzentrum, D-82152 Martinsried, Germany */
/* email: krasel at alf.biochem.mpg.de                 fax: +49 89 8578 3795 */
/* "People are DNA's way of making more DNA." (Edward O. Wilson, 1975)    */



More information about the Methods mailing list