detergent removal help!
scott_basehore at stratagene.com
scott_basehore at stratagene.com
Wed Jun 22 18:34:24 EST 1994
I have purified an enzyme in a buffer with 0.1% NP-40 and
0.1% Twn-20. In order to grow crystals of my enzyme,
suitable for X-ray crystallography, I need to remove these
two detergents. Dialysis won't work as the detergents are
above the critical conc. for the formation of micelles. I
have tried an Extracti-gel D (Pierce) column, but the
solution still foams when agitated (low-tech assay). I am
fairly sure that my enzyme alone will not foam at its
present concentration. Does anyone know a method for
removing these detergents? How might one assay for the
presence of detergents?
"I am approaching the hypotenuse of my angst"
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