Black DNA from earthworms
mccallo at rand
mccallo at rand
Wed Jun 22 18:03:38 EST 1994
<sjames at miu.edu>, Iowa State University, Ames, Iowa writes:
>I would like to know if anyone has encountered a similar phenomenon with
>other critters. When we do any of several standard DNA preps, we get a
>very dark brown to black pellet that turns out to be DNA.
*cut*
Plasmid DNA from Bacillus megaterium is often yellow-orange; also does not cut
well. Even with repeated CsCl bandings it would still be colored. I found
that Qiagen columns fix the problem; its white and it cuts.
Owen McCall
Abbott Laboratories
Abbott Park, IL USA
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