help lambda-gt11 PCR

Carlisle Landel landel at helios
Wed Jun 22 10:00:35 EST 1994


The trick for directly PCRing lambda clones is to pick the plaque into
water, not SM.  All that Mg in the SM (and other phage storage media)
*really* mess up the PCR.  We pick a plaque to 100 ul H20 and then use
10 ul for PCR.

Good Luck!

Carlisle




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