Pbluescript I map

Francis Ouellette francis at borduas.nlm.nih.gov
Wed Jun 22 22:53:54 EST 1994


: >Nelson Salazar (N.SALAZAR at lshtm.ac.uk) wrote:
: >
: >:                 simple questions. Where can I find the map of the 
: >: vector pBluescript I ?? Is there a SacII site in the cloning site of 
: >: this vector ?? What is the difference between this one and the 
: >: pBluescript II SK ??

something else you can do is get the sequences from GenBank
(the retrieve server at NCBI is one way) and get the sequences
for all the pBlueScript vectors.  Here are the accession
numbers:

LOCUS       ARBL2KSM     2961 bp    DNA   Circular  SYN       23-NOV-1993
DEFINITION  pBluescript II KS(-) vector DNA, phagemid excised from lambda ZAPII
ACCESSION   X52329
//
LOCUS       ARBL2KSP     2961 bp    DNA   Circular  SYN       23-NOV-1993
DEFINITION  pBluescript II KS(+) vector DNA, phagemid excised from lambda ZAPII
ACCESSION   X52327
//
LOCUS       ARBL2SKM     2961 bp    DNA   Circular  SYN       23-NOV-1993
DEFINITION  pBluescript II SK(-) vector DNA, phagemid excised from lambda ZAPII
ACCESSION   X52330 S52394
//
LOCUS       ARBL2SKP     2961 bp    DNA   Circular  SYN       23-NOV-1993
DEFINITION  pBluescript II SK(+) vector DNA, phagemid excised from lambda ZAPII
ACCESSION   X52328
//
LOCUS       ARBLKSM      2958 bp    DNA   Circular  SYN       23-NOV-1993
DEFINITION  pBluescript KS(-) vector DNA, phagemid excised from lambda ZAP
ACCESSION   X52326
//
LOCUS       ARBLKSP      2958 bp    DNA   Circular  SYN       23-NOV-1993
DEFINITION  pBluescript KS(+) vector DNA, phagemid excised from lambda ZAP
ACCESSION   X52331
//
LOCUS       ARBLSKM      2958 bp    DNA   Circular  SYN       23-NOV-1993
DEFINITION  pBluescript SK(-) vector DNA, phagemid excised from lambda ZAP
ACCESSION   X52324 S52394
//
LOCUS       ARBLSKP      2958 bp    DNA   Circular  SYN       23-NOV-1993
DEFINITION  pBluescript SK(+) vector DNA, phagemid excised from lambda ZAP
ACCESSION   X52325
//


To use the retrieve server, send 'help' to
retrieve at ncbi.nlm.nih.gov

and in the return mail (a few minutes) you will get info on
how to use the ncbi retrieve server an get these sequences.

An alternative is our Web server (see URL below) and the
entrez and network entrez software.  For more info on these,
send email to:

info at ncbi.nlm.nih.gov


regards,

francis   

--
| B.F. Francis Ouellette  
|
| francis at ncbi.nlm.nih.gov   



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